Ion Trap
Ion trap is a combination of electric or magnetic fields used to capture ions in a region of a vacuum system or tube. Some of the scientific uses of ion trap instrument can be seen in fields of mass spectrometry and in trapping ions while the ion's quantum state is manipulated.
Types of Ion Traps
There are basically two types of ion traps that are commonly used by the science community. These are-
Penning Trap; and
Paul Trap also called Quadrupole Ion Trap. However, these two ion trap instrument are not the only types of ion traps. There are other ion traps too like the
Kingdon trap. Paul trap is generally used for scientific studies of quantum state manipulation. An ion trap mass spectrometer may incorporate within itself a Penning trap, a Paul trap or a Kingdon trap. Some types of mass spectrometers may also use a
linear quadrupole ion trap.
Ion Trap as Biotech Lab Equipment
The Ion Trap instrument today is used widely as a
biotechnology lab equipment. It is a significant laboratory apparatus in the fields of proteomics and metabolomics. Some of the applications of ion traps in the biotechnology researches include:
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Protein identification, profiling and detailed characterization
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Protein and peptide analysis to determine MW
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Metabolite identification and profiling
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General chemistry LC/MSn
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Protein post-translational modification
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Protein structure characterization
Ion Trap Mass Spectrometry
Ion Trap Mass Spectrometer
Mass spectrometry is a powerful technique for chemical analysis used to identify unknown compounds; quantify known compounds; and to elucidate molecular structure. Some typical applications of mass spectrometry include:
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Environmental monitoring and analysis of soil, water and air pollutants, water quality, etc.
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Geochemistry applications like age determination, soil and rock composition, oil and gas surveying
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Chemical and petrochemical industry applications involving quality control
There are certain applications of mass spectrometry that are specific to biotechnology. These include:
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Identifying structures of biomolecules like carbohydrates, nucleic acids etc.
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Sequence biopolymers like proteins and oligosaccharides
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Determining drug metabolic pathways
Thus, mass spectrometry provides valuable information to many professionals like chemists, biologists, physicians, astronomers, environmental health specialists, biotechnology researchers etc. Ion trap mass spectrometer is thus a very important laboratory apparatus.
Ion Trap Mass Spectrometer
Mass Spectrometer Structure
A
mass spectrometer can be called a "Molecule Smasher" which measures molecular and atomic masses as well as other properties of molecules. There are different types of mass spectrometers with there own sets of benefits, limitations and applications. However, the feature which is similar in all types of mass spectrometers is there structure that consists of sections linked together with each other. These parts of mass spectrometer are:
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Sample Inlet;
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Ion Source;
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Mass Analyzer;
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Detector;
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Data System; and
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Computer Control.
The
Quadrupole Ion Trap Mass Spectrometer (ITMS) has come up as an excellent tool for biomolecular analysis in the present times. A quadrupole ion trap instrument has a small size equal to that of a tennis ball. Its size is inversely proportional to its versatility. The core of this ion trap instrument is formed of three hyperbolic electrodes, consisting of a ring and two endcaps. It is basically a laboratory apparatus that is widely used as
physics lab equipment.
Other Biotechnology Equipment