Electron
Microscopes are popularly used scientific and analytical instruments using a
beam of highly energized electrons to examine objects.
Working Principle
his special type of microscope makes use of electrons instead of light waves
to create magnified images. Three dimensional figures are shown by these
microscope and with much higher magnifications. The images are black &
white because of the absence of light waves.
Samples to be examined or viewed must be prepared carefully so as to enable
them to withstand vacuum inside the microscope. The biological specimens
should be able to conduct electricity because they are illuminated with
electrons. These equipment give the details of microorganisms and other
minute objects.
Magnifying Process
- A stream of electrons is created and accelerated towards the specimen
with the help of a positive electrical potential.
- This stream is focused and then formed into a monochromatic beam
using metal apertures and magnetic lenses.
- The beam is then focused onto the sample / specimen with the help of
a magnetic lens
- This interaction affects the beam and then transformed into an image,
which can be clearly seen.
Use of Electron Microscope
- Educational Laboratories
- Biotechnology
- Botany
- Research Laboratory
- Pathological Laboratory
- Clinical Laboratory
